| 质粒名称: | AAVS1 T2 CRIPR in pX330 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 72833 |
| 存储实验室: | Masato Kanemaki |
|
载体骨架基本信息 |
|
| 载体名称: | pX330 |
| 载体抗性: | 氨苄青霉素 |
| 载体类型: | 哺乳细胞表达,CRISPR |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Cas9 and gRNA for targeting the AAVS1 locus in human cells |
| 物种来源: | H. sapiens (human) |
| 启动子 | U6 promoter |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BbsI（克隆过程中销毁） |
| 3' 克隆位点: | BbsI（克隆过程中销毁） |
| 5' 测序引物 | aggctgttagagagataattgg |
|
文献引用方法 |
|
| 材料和方法部分: | AAVS1 T2 CRIPR in pX330 was a gift from Masato Kanemaki (Addgene plasmid # 72833 ; http://n2t.net/addgene:72833 ; RRID:Addgene_72833) |
| 参考文献部分: | Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT. Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001. Epub 2016 Mar 24. 10.1016/j.celrep.2016.03.001 PubMed 27052166 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS AAVS1 T2 CRIPR in pX330 8507 bp ds-DNA circular SYN 16-MAY-2019
DEFINITION CRISPR/Cas to target the AAVS1 locus in human cells.
ACCESSION .
VERSION .
KEYWORDS AAVS1 T2 CRIPR in pX330
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8507)
AUTHORS Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
TITLE Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron
Tagging with Short Homology Donors.
JOURNAL Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
Epub 2016 Mar 24.
PUBMED 27052166
REFERENCE 2 (bases 1 to 8507)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 16, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8507
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..241
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 1..21
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 172..191
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 270..345
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
enhancer 442..727
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
intron 1007..1234
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
regulatory 1246..1255
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1255..1320
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 1327..1347
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1372..5472
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5473..5520
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
primer_bind complement(5548..5565)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5554..5761
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 5770..5910
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 5770..5899
/label=AAV2 ITR
rep_origin 5985..6440
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6072..6091)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6282..6303
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6457..6476)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6576..6598
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6636..6654)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6722..6826
/gene="bla"
/label=AmpR promoter
CDS 6827..7687
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7045..7064)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7858..8446
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8347..8366
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
241 cgaaacaccg gggccactag ggacaggatg ttttagagct agaaatagca agttaaaata
301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag
361 ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag
421 acaaatggct ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc
481 gcccaacgac ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg
541 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
601 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca
661 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
721 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
781 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
841 gggggggggg cgcgcgccrg gsggggsggg ggggggsggg ggggggggsg gggsgrggsg
901 grgrggkgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
961 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
1021 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
1081 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
1141 gctgagcaag aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta
1201 cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac catggactat
1261 aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga tgacgataag
1321 atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag
1381 tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag
1441 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
1501 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
1561 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
1621 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
1681 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
1741 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
1801 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
1861 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
1921 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
1981 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat
2041 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg
2101 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg
2161 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac
2221 cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac
2281 atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga
2341 tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct
2401 gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac
2461 ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac
2521 ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc
2581 ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg
2641 cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg
2701 accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg
2761 atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag
2821 ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac
2881 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg
2941 accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag
3001 aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg
3061 aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa
3121 gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag
3181 gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca
3241 ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac
3301 gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg
3361 aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag
3421 tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt
3481 aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt
3541 gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg
3601 gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc
3661 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc
3721 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc
3781 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg
3841 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag
3901 agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg
3961 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg
4021 cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag
4081 agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc
4141 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac
4201 gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc
4261 gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc
4321 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg
4381 gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag
4441 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac
4501 tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag
4561 acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg
4621 aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc
4681 ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag
4741 gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg
4801 gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg
4861 gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc
4921 aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc
4981 gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac
5041 gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag
5101 ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac
5161 tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac
5221 gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag
5281 caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc
5341 aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac
5401 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag
5461 ctgggaggcg acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag
5521 taagaattcc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
5581 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
5641 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
5701 gtggggtggg gcaggacagc aagggggagg attgggaaga gaatagcagg catgctgggg
5761 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
5821 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
5881 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
5941 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
6001 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
6061 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
6121 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
6181 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
6241 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
6301 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
6361 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
6421 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
6481 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
6541 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
6601 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
6661 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
6721 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
6781 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
6841 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
6901 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
6961 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
7021 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
7081 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
7141 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
7201 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
7261 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
7321 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
7381 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
7441 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
7501 tggctggttt attgctgata aatctggagc cggtgagcgt ggaagccgcg gtatcattgc
7561 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
7621 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
7681 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
7741 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
7801 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
7861 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
7921 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
7981 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
8041 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
8101 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
8161 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
8221 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
8281 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
8341 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
8401 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
8461 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
